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anti eomes antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti eomes antibody
    <t>Early</t> <t>mesodermal</t> commitment is accompanied by reduced sulfide metabolic flux and altered protein persulfidation that maintains stemness. (A) 34 S cystine derived polysulfides normalized to iPSCs. (B) False color images showing the intensity of the Daz2:Cy5 persulfidation signal iPSCs and in the three germ layers. bar = 10 μm. Fire pseudocoloring was used to distinguish the signal intensity. F-actin: black, NBF–Cl: red. (C) Volcano plot showing persulfidated proteins in iPSCs or cells directed to the mesodermal lineage. (D) OCT4 activity reporter assay in iPSCs treated with solvent (Sol.) or with 5 μmol/L Na 2 S 3 for 24 h. (E) β-catenin stability in mesodermal cells treated with solvent (Sol.) or with 5 μmol/L Na 2 S 3 for 24 h and representative immunoblot at 24 h. (F) Percentage (%) of <t>EOMES-positive</t> live cells in samples treated as in panel E. 3 iPSC lines were used in 2–3 technical replicates. Graphs include the average of all experiments per line. Paired Student's t-test (C, D, F), One-way ANOVA, Tukey's multiple comparison test (A, B), Two-way ANOVA, Tukey's multiple comparison test (E).
    Anti Eomes Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 887 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+eomes+antibody/HSP90+Rabbit+mAb/pmc12721145-79-8-10
    Average 97 stars, based on 887 article reviews
    anti eomes antibody - by Bioz Stars, 2026-10
    97/100 stars

    Images

    1) Product Images from "Persulfidation alters gene regulatory programs and promotes endothelial specification"

    Article Title: Persulfidation alters gene regulatory programs and promotes endothelial specification

    Journal: Redox Biology

    doi: 10.1016/j.redox.2025.103926

    Early mesodermal commitment is accompanied by reduced sulfide metabolic flux and altered protein persulfidation that maintains stemness. (A) 34 S cystine derived polysulfides normalized to iPSCs. (B) False color images showing the intensity of the Daz2:Cy5 persulfidation signal iPSCs and in the three germ layers. bar = 10 μm. Fire pseudocoloring was used to distinguish the signal intensity. F-actin: black, NBF–Cl: red. (C) Volcano plot showing persulfidated proteins in iPSCs or cells directed to the mesodermal lineage. (D) OCT4 activity reporter assay in iPSCs treated with solvent (Sol.) or with 5 μmol/L Na 2 S 3 for 24 h. (E) β-catenin stability in mesodermal cells treated with solvent (Sol.) or with 5 μmol/L Na 2 S 3 for 24 h and representative immunoblot at 24 h. (F) Percentage (%) of EOMES-positive live cells in samples treated as in panel E. 3 iPSC lines were used in 2–3 technical replicates. Graphs include the average of all experiments per line. Paired Student's t-test (C, D, F), One-way ANOVA, Tukey's multiple comparison test (A, B), Two-way ANOVA, Tukey's multiple comparison test (E).
    Figure Legend Snippet: Early mesodermal commitment is accompanied by reduced sulfide metabolic flux and altered protein persulfidation that maintains stemness. (A) 34 S cystine derived polysulfides normalized to iPSCs. (B) False color images showing the intensity of the Daz2:Cy5 persulfidation signal iPSCs and in the three germ layers. bar = 10 μm. Fire pseudocoloring was used to distinguish the signal intensity. F-actin: black, NBF–Cl: red. (C) Volcano plot showing persulfidated proteins in iPSCs or cells directed to the mesodermal lineage. (D) OCT4 activity reporter assay in iPSCs treated with solvent (Sol.) or with 5 μmol/L Na 2 S 3 for 24 h. (E) β-catenin stability in mesodermal cells treated with solvent (Sol.) or with 5 μmol/L Na 2 S 3 for 24 h and representative immunoblot at 24 h. (F) Percentage (%) of EOMES-positive live cells in samples treated as in panel E. 3 iPSC lines were used in 2–3 technical replicates. Graphs include the average of all experiments per line. Paired Student's t-test (C, D, F), One-way ANOVA, Tukey's multiple comparison test (A, B), Two-way ANOVA, Tukey's multiple comparison test (E).

    Techniques Used: Derivative Assay, Activity Assay, Reporter Assay, Solvent, Western Blot, Comparison

    Related Articles

    Staining:

    Article Title: Persulfidation alters gene regulatory programs and promotes endothelial specification
    Article Snippet: Live cells were identified using a viability dye (e.g. SYTOXTM Red, Thermo Fisher, Cat. #S34859) prior to fixation and permeabilization with BD Cytofix/Cytoperm buffer (BD Biosciences). .. Intracellular staining for mesodermal specification was performed using anti-EOMES antibody (Cell Signaling Technology, Cat. #14-4877-82, clone WD1928, human reactive). .. Data acquisition and cell sorting were carried out on a BD FACSAria II (BD Biosciences) and analysis was performed using FlowJo V10 (TreeStar).

    Article Title: Persulfidation alters gene regulatory programs and promotes endothelial specification.
    Article Snippet: Live cells were identified using a viability dye (e.g. SYTOXTM Red, Thermo Fisher, Cat. #S34859) prior to fixation and permeabilization with BD Cytofix/Cytoperm buffer (BD Biosciences). .. Intracellular staining for mesodermal specification was performed using anti-EOMES antibody (Cell Signaling Technology, Cat. #14-4877-82, clone WD1928, human reactive). .. Data acquisition and cell sorting were carried out on a BD FACSAria II (BD Biosciences) and analysis was performed using FlowJo V10 (TreeStar).



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    Image Search Results


    Early mesodermal commitment is accompanied by reduced sulfide metabolic flux and altered protein persulfidation that maintains stemness. (A) 34 S cystine derived polysulfides normalized to iPSCs. (B) False color images showing the intensity of the Daz2:Cy5 persulfidation signal iPSCs and in the three germ layers. bar = 10 μm. Fire pseudocoloring was used to distinguish the signal intensity. F-actin: black, NBF–Cl: red. (C) Volcano plot showing persulfidated proteins in iPSCs or cells directed to the mesodermal lineage. (D) OCT4 activity reporter assay in iPSCs treated with solvent (Sol.) or with 5 μmol/L Na 2 S 3 for 24 h. (E) β-catenin stability in mesodermal cells treated with solvent (Sol.) or with 5 μmol/L Na 2 S 3 for 24 h and representative immunoblot at 24 h. (F) Percentage (%) of EOMES-positive live cells in samples treated as in panel E. 3 iPSC lines were used in 2–3 technical replicates. Graphs include the average of all experiments per line. Paired Student's t-test (C, D, F), One-way ANOVA, Tukey's multiple comparison test (A, B), Two-way ANOVA, Tukey's multiple comparison test (E).

    Journal: Redox Biology

    Article Title: Persulfidation alters gene regulatory programs and promotes endothelial specification

    doi: 10.1016/j.redox.2025.103926

    Figure Lengend Snippet: Early mesodermal commitment is accompanied by reduced sulfide metabolic flux and altered protein persulfidation that maintains stemness. (A) 34 S cystine derived polysulfides normalized to iPSCs. (B) False color images showing the intensity of the Daz2:Cy5 persulfidation signal iPSCs and in the three germ layers. bar = 10 μm. Fire pseudocoloring was used to distinguish the signal intensity. F-actin: black, NBF–Cl: red. (C) Volcano plot showing persulfidated proteins in iPSCs or cells directed to the mesodermal lineage. (D) OCT4 activity reporter assay in iPSCs treated with solvent (Sol.) or with 5 μmol/L Na 2 S 3 for 24 h. (E) β-catenin stability in mesodermal cells treated with solvent (Sol.) or with 5 μmol/L Na 2 S 3 for 24 h and representative immunoblot at 24 h. (F) Percentage (%) of EOMES-positive live cells in samples treated as in panel E. 3 iPSC lines were used in 2–3 technical replicates. Graphs include the average of all experiments per line. Paired Student's t-test (C, D, F), One-way ANOVA, Tukey's multiple comparison test (A, B), Two-way ANOVA, Tukey's multiple comparison test (E).

    Article Snippet: Intracellular staining for mesodermal specification was performed using anti-EOMES antibody (Cell Signaling Technology, Cat. #14-4877-82, clone WD1928, human reactive).

    Techniques: Derivative Assay, Activity Assay, Reporter Assay, Solvent, Western Blot, Comparison